PA (Physician Assistant) students experience a high degree of burnout, and mental health indicators have been shown to worsen during clinical year of training. However, stigma and lack of awareness of resources remain barriers to help-seeking, and no published guideline currently exists to proactively support PA students' mental health. We used this study to evaluate the effectiveness of Mental Health First Aid (MHFA) training for PA students regarding mental health literacy and stigma, awareness of resources, and practical application.