MINITAB Practice: Designing a Factorial Experiment
Designed a factorial experiment using Minitab to help troubleshoot IHC (immunohistochemistry) staining problems with tissues falling off the slides.
The two factors of this experiment was sample types based on position of slides in slide box and blocking types based on the blocking solution used for non-specific staining.
Each factor contained two levels. For sample types, it is Urobatis Tg upper right and Urobatis Tg lower left. For blocking types, it is new blocking solution and old blocking solution.
The idea was to subject the Urobatis Tg tissues to different blocking solutions to see whether neural crest cells could be identified by a fluorescent microscope. If the new blocking solution lead to more cells and the old blocking solution showed no cells, then the old blocking solution would be the problem. If no cells were detected using both the new blocking solution or the old blocking solution, then the most probable issue would be the tissue fixation problem.
This experiment was initially created to help troubleshoot in the lab but ended up not being considered. However, my initial suggestion of the old blocking solution being too old (it was ordered in 2021 and sat in the fridge for over a year) turned out to be the culprit which lead to a new blocking solution being made from scratch and the tissues staying on the slide.
The two factors of this experiment was sample types based on position of slides in slide box and blocking types based on the blocking solution used for non-specific staining.
Each factor contained two levels. For sample types, it is Urobatis Tg upper right and Urobatis Tg lower left. For blocking types, it is new blocking solution and old blocking solution.
The idea was to subject the Urobatis Tg tissues to different blocking solutions to see whether neural crest cells could be identified by a fluorescent microscope. If the new blocking solution lead to more cells and the old blocking solution showed no cells, then the old blocking solution would be the problem. If no cells were detected using both the new blocking solution or the old blocking solution, then the most probable issue would be the tissue fixation problem.
This experiment was initially created to help troubleshoot in the lab but ended up not being considered. However, my initial suggestion of the old blocking solution being too old (it was ordered in 2021 and sat in the fridge for over a year) turned out to be the culprit which lead to a new blocking solution being made from scratch and the tissues staying on the slide.
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