This project describes the implementation of Eco-Driving in transit buses using the Eco-Speed Control (ESC) algorithm. The ESC algorithm is used as an optimization tool to reduce the fuel consumption of an individual vehicle using vehicle-to-vehicle and vehicle-to-infrastructure communication to receive data from surrounding vehicles and downstream signalized intersections to develop a fuel efficient trajectory. This trajectory is communicated to the driver as an audio alert. Three different scenarios were tested, namely 1) an uninformed driver, 2) an informed driver with knowledge of the time to the next signal indication change and 3) an informed driver with knowledge of the target speed that is calculated by the ESC algorithm. A field test including three scenarios was conducted on the Virginia Tech Transportation Institute’s Smart Road. The data from 48 trips by three different participants were collected in the preliminary field test. The test results demonstrated that in comparison to the uniformed driver, the informed driver in scenario 3 saves 36.3% and 10.8% in fuel consumption for the downhill and uphill approaches, respectively. In addition, the informed driver in scenario 3 saves 8.2% and 7.1% in travel time for the downhill and uphill directions, respectively. The preliminary field testing results demonstrate that the proposed system provides savings of fuel and travel time for buses while traversing signalized intersections.